Structural proteomics: prospects for high throughput sample preparation.
نویسندگان
چکیده
Structural proteomics: prospects for high throughput sample preparation Dinesh Christendat, Adelinda Yee, Akil Dharamsia,y, Yuval Kluger, Mark Gerstein, Cheryl H. Arrowsmith*, Aled M. Edwards Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, 610 University Avenue, Toronto, Ontario, Canada M5G2M9 C.H. Best Institute, Banting and Best Department of Medical Research, University of Toronto, 610 University Avenue, Toronto, Ontario, Canada M5G2M9 Department of Molecular Biophysics and Biochemistry, Yale University, Connecticut, USA
منابع مشابه
A high-throughput sample preparation method for cellular proteomics using 96-well filter plates.
A high-throughput sample preparation protocol based on the use of 96-well molecular weight cutoff (MWCO) filter plates was developed for shotgun proteomics of cell lysates. All sample preparation steps, including cell lysis, buffer exchange, protein denaturation, reduction, alkylation and proteolytic digestion are performed in a 96-well plate format, making the platform extremely well suited fo...
متن کاملAutomation for LC/MS Sample Preparation: High Throughput In-Solution Digestion and Peptide Cleanup Enabled by the Agilent AssayMAP Bravo Platform
Protein and peptide sample preparation for LC/MS remains entrenched in workflows reliant upon serial manual manipulations resulting in protocols lacking in throughput, scalability, and transferability. These workflows are often dependent on highly skilled practitioners to achieve acceptable reproducibility, or, in many cases, large variability is simply accepted as intrinsic to the workflow. To...
متن کاملEfficient sample processing for proteomics applications—Are we there yet?
The ability to solubilize and digest protein extracts and recover peptides with high efficiency is of paramount importance in proteomics. A novel proteomic sample preparation protocol by Krijgsveld and colleagues (Hughes et al, 2014) provides significant advantages by enabling all sample processing steps to be carried out in a single tube to minimize sample losses, thereby enhancing sensitivity...
متن کاملSolid-Phase Extraction Strategies to Surmount Body Fluid Sample Complexity in High-Throughput Mass Spectrometry-Based Proteomics
For large-scale and standardized applications in mass spectrometry- (MS-) based proteomics automation of each step is essential. Here we present high-throughput sample preparation solutions for balancing the speed of current MS-acquisitions and the time needed for analytical workup of body fluids. The discussed workflows reduce body fluid sample complexity and apply for both bottom-up proteomic...
متن کاملAn integrated digital microfluidic chip for multiplexed proteomic sample preparation and analysis by MALDI-MS.
To realize multiplexed sample preparation on a digital microfluidic chip for high-throughput Matrix Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS), several fluidic functions need to be integrated. These include the generation of multiple droplets from a reservoir and parallel in-line sample purification. In this paper, we develop two critical new functions in handling protein...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Progress in biophysics and molecular biology
دوره 73 5 شماره
صفحات -
تاریخ انتشار 2000